| BCRJ Code | 0266 |
| Cell Line | SK-ES-1 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Bone |
| Morphology | Epithelial |
| Disease | Sarcoma (Anaplastic Osteosarcoma Or Ewing'S Sarcoma) |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 18 Year / Caucasian |
| DNA Profile | Amelogenin: X,Y CSF1PO: 11 D13S317: 8,9 D16S539: 11 D5S818: 12 D7S820: 10,11 THO1: 6,9.3 TPOX: 8 vWA: 14,17 |
| Tumor Formation: | Yes, in nude mice, forms small cell malignant tumor consistent with Ewing sarcoma |
| Products | Antigen expression: Blood Type A; Rh+ |
| Biosafety | 1 |
| Addtional Info | Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines. Sera from normal donors were also cytotoxi |
| Culture Medium | McCoy's 5A Medium is modified to contain fetal bovine serum to a final concentration of 15%. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:2 to 1:5 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975. 22423: Bloom ET. Further definition by cytotoxicity tests of cell surface antigens of human sarcomas in culture. Cancer Res. 32: 960-967, 1972. PubMed: 4502173 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 |
| Depositors | Caroline Brunetto - Instituto do Câncer Infantil do RS |
| Cellosaurus | CVCL_0627 |
