| BCRJ Code | 0382 |
| Cell Line | SW 1353 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Bone |
| Morphology | Fibroblast |
| Disease | Chondrosarcoma |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 72 Year / Caucasian |
| Derivation | The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian. |
| Applications | Transfection host |
| Biosafety | 1 |
| Culture Medium | Leibovitz's L-15 Medium and fetal bovine serum to a final concentration of 10%. The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. |
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:02 |
| Culture Conditions | Atmosphere: air, 100%; carbon dioxide (CO2), 0% |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| Depositors | Daniele Ribeiro de Araujo - Universidade Federal do ABC |
| Cellosaurus | CVCL_0543 |
