| BCRJ Code | 0231 |
| Cell Line | T24 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Urinary Bladder |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Transitional Cell Carcinoma |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 81 Year / Caucasian |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 9 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,11 vWA: 17 |
| Tumor Formation: | Yes, in hamster cheek pouch No, in nude mice |
| Products | tumor specific antigen |
| Biosafety | 1 |
| Addtional Info | Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. Contains the ras (H-ras) oncogene. |
| Culture Medium | McCoy's 5A Medium is modified to contain 2 mM L-glutamine and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Cells have a 19 hour generation time. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:3 to 1:8 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Bellet D , et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. Bubenik J , et al. Cellular immunity t |
| Depositors | Fernanda Bueno Morrone Pontífica Universidade Católica do Rio Grande do Sul |
| ATCC | HTB-4 |
| Cellosaurus | CVCL_0554 |
