| BCRJ Code | 0372 |
| Cell Line | TE-6 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Esophagus |
| Morphology | Epithelial-Like |
| Disease | Squamous Carcinoma |
| Growth Properties | Adherent |
| Sex | Male |
| Biosafety | 2 |
| Addtional Info | Highly differentiated squamous carcinoma (esophagus), not transplantable to nude mouse. Progeny were cured by Treatments with BM Cycline and MC210. TKG0257 (Deposited from Tohoku Univ.). |
| Culture Medium | RPMI 1640 with 10% of fetal bovine serum. |
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Subculturing Medium Renewal | Twice a week |
| Subculturing Subcultivation Ratio | 1:8 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
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| Depositors | Jolie Kiemlian Kwee - Instituto Nacional de Câncer |
| Cellosaurus | CVCL_1765 |
