| BCRJ Code | 0234 |
| Cell Line | THP-1 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Peripheral Blood |
| Cell Type | Monocyte |
| Morphology | Monocyte |
| Disease | Acute Monocytic Leukemia |
| Growth Properties | Suspension |
| Sex | Male |
| Age/Ethinicity | 1 Year / |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 13 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 8,9.3 TPOX: 8,11 vWA: 16 |
| Products | lysozyme |
| Biosafety | 1 |
| Addtional Info | The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin. [58053] Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). [22193] |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 10e5 viable cells/mL. Subculture when cell concentration reaches 8x10e5 cells/mL. NOUTE: Do not allow the cell concentration to exceed 1 x 10e6 cells/mL |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 22193: Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641 22285: Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclon |
| Depositors | ANDRESSA COOPE DOS SANTOS; UNICAMP |
| ATCC | TIB-202 |
| Cellosaurus | CVCL_0006 |
