| BCRJ Code | 0235 |
| Cell Line | Toledo |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Peripheral Blood |
| Cell Type | B Lymphocyte |
| Morphology | Lymphoblast |
| Disease | Diffuse Large Cell Lymphoma; Non-Hodgkin'S B Cell Lymphoma |
| Growth Properties | Suspension |
| Sex | Female |
| Age/Ethinicity | ADULT / White |
| Derivation | Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL). [53289] |
| Applications | This cell line is a model system for studying non-Hodgkin lymphomas. |
| DNA Profile | D5S818:10,12 D7S820: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 Amelogenin: X CSF1PO: 12 D13S317:11 D16S539:9 |
| Biosafety | 1 |
| Addtional Info | Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain. Though the morphology of the cells resembles Burkitt's lymphoma, the cells lack the typical chromosomal translocations of Burkitt's lymphoma. The karyotype does exhibit multiple chromosomal aberrations. The cells do not express surface or cytoplasmic immunoglobulin. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 10e5 viable cells/mL. Maintain cultures at cell concentrations between 3 x 10e5 and 3 x 10e6 viable cells/mL. Population Doubling Time: 24 to 30 hrs |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Depositors | RAQUEL MAIA; INCA |
| Cellosaurus | CVCL_3611 |
