| BCRJ Code | 0237 |
| Cell Line | TRFK-4 |
| Species | Rattus norvegicus |
| Vulgar Name | Rat |
| Tissue | Hematopoietic |
| Cell Type | Hybridoma: B Lymphocyte |
| Morphology | Lymphoblast |
| Growth Properties | Suspension |
| Products | Immunoglobulin; monoclonal antibody; IL-5: ab against; interleukin-5 |
| Biosafety | 1 |
| Addtional Info | This hybridoma secretes neutraling antibody against murine interleukin-5 (IL-5). The origin of this cell line should be acknowledge in all relevant publications. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 1 mM sodium pyruvate, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures from 1x10 exp5 to 1x10 exp6 cells/mL. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | J. Immunol. 141: 1576-81, 1988. |
| Depositors | Robert Coffman, Dnax Research Institute Of Molecular And Cellular Biology |
| Cellosaurus | CVCL_D154 |
