BCRJ Code | 0239 |
Cell Line | U-138 MG |
Species | Homo sapiens |
Vulgar Name | Human |
Tissue | Brain |
Morphology | Polygonal |
Disease | Glioblastoma |
Growth Properties | Adherent |
Sex | Male |
Age/Ethinicity | 47 Year / Caucasian |
DNA Profile | Amelogenin: X,Y CSF1PO: 12 D13S317: 9,11 D16S539: 12,13 D5S818: 11 D7S820: 9 THO1: 6 TPOX: 8 vWA: 18 |
Tumor Formation: | NO |
Biosafety | 1 |
Culture Medium | Dulbecco's Modified Eagle's Medium with 2 mM L-glutamine and fetal bovine serum to a final concentration of 10%. |
Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
Subculturing Medium Renewal | 2 to 3 times per week |
Subculturing Subcultivation Ratio | 1:4 to 1:8 |
Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
References | Beckman G , et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines |
Depositors | Ana Maria Oliveira Battastini, Universidade Federal Do Rio Grande Do Sul. |
Cellosaurus | CVCL_0020 |
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