| BCRJ Code | 0304 |
| Cell Line | U-2 OS |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Bone |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Osteosarcoma |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 15 Year / Caucasian |
| Derivation | J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl. |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 6,9.3 TPOX: 11,12 vWA: 14,18 |
| Products | Antigen expression: Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) Receptor expression: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II) Genes Expressed: osteosacoma derived growth factor (ODGF),Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
| Biosafety | 1 |
| Addtional Info | RECEPTORES EXPRESSED: INSULIN-LIKE GROWTH FACTOR I (IGF-I); INSULIN-LIKE GROWTH FACTOR II(IGF-II) |
| Culture Medium | McCoy's 5A Medium is modified to contain 2 mM L-glutamine and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. T-75 flasks are recommended for subculturing this product. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. |
| Depositors | SILVIA REGINA CAMINADA DE TOLEDO - Grupo de Apoio ao Adolescente e Criança com Câncer |
| Cellosaurus | CVCL_0042 |
