| BCRJ Code | 0240 |
| Cell Line | U266B1 [U266] |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Peripheral Blood |
| Cell Type | B Lymphocyte |
| Morphology | Lymphoblast |
| Disease | Myeloma; Plasmocytoma |
| Growth Properties | Suspension |
| Age/Ethinicity | 53 Year / |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 12,13 D13S317: 12 D16S539: 10 D5S818: 11,12 D7S820: 11,12 THO1: 5,7 TPOX: 8 vWA: 17 |
| Products | immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6) |
| Biosafety | 1 |
| Addtional Info | U266 cells have been reported to produce human IL-6. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate and 15% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10e5 cells/mL and maintain between 1 x 10e5 and 1 x 10e6 cells/mL. T-75 flasks are recommended for subculturing this cell line. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 1180: Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745 22377: Kawano M, et al. Autocrine generation and requirement |
| Depositors | PRISCILA SEGGES; INCA |
| Cellosaurus | CVCL_0566 |
