| BCRJ Code | 0243 |
| Cell Line | UMR-106 |
| Species | Rattus norvegicus |
| Vulgar Name | Rat |
| Tissue | Bone |
| Morphology | Epithelial |
| Disease | Osteosarcoma |
| Growth Properties | Adherent |
| Derivation | Both the original sarcoma and the cloned line were developed by T.J. Martin at the University of Sheffield. The UMR-106 cell line is a clonal derivative of a transplantable rat osteosarcoma that had been induced by injection of radiophosphorous (32P). |
| Biosafety | 1 |
| Addtional Info | The cells are responsive to PTH, prostaglandins and bone resorbing steroids. Activation of protein kinase C inhibits ATP induced increases in intracellular calcium levels. |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 2 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and 10% of fetal bovine serum. |
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:4 to 1:8 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Nature (Lond. ) 260: 436-438, 1976; Eur. J. Cancer 15: 1151-1158, 1979; Clin. Orthop. Rel. Res. 140: 247-254, 1979; FEBS Lett. 115: 139-142,1980; Cancer Res. 43: 4308-4315, 1983; Methods Enzymol. 145: 324-336; 1987; |
| Depositors | Dr. Willian George Goodman, Deparment of Radiololgy, UCLA School of Medicine through Dr. Maria Eugênia Leite Duarte, Universidade Federal Fluminense. |
| Cellosaurus | CVCL_3617 |
