| BCRJ Code | 0251 |
| Cell Line | XR63-IL3 |
| Species | Mus musculus |
| Vulgar Name | Mouse |
| Tissue | Hematopoietic |
| Cell Type | Myeloid |
| Morphology | Lymphoblast |
| Disease | Myeloma |
| Growth Properties | Suspension |
| Derivation | XR63-IL3 was derived from X63AG-654 myeloma cell line. |
| Products | Interleukin-3; IL-3 |
| Biosafety | 1 |
| Addtional Info | It was transformed py pBV-1MTHA vector containing IL-3 cDNA cloned by Noma ( Nature, 1986, 319:640). Transformants produce mouse IL-3 in titres higher than those produced by concanavalin A stimulating T cells. This IL-3 produced the 3H-Thymidine uptake of T-cell line, a mast cell line and B leukemia cells, and enhanced the productions of IgG1 by B-cells. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 1 mM sodium pyruvate, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10e5 cells/mL and maintain between 2 X 10e5 and 10e6 cells/mL. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Eur J Immunol 18:97-104, 1988. |
| Depositors | Dr. Fritz Melchers, Basel Institute for Immunology, Basel Switzeland. |
| Cellosaurus | CVCL_M352 |
